Our research is aimed at understanding molecular mechanisms of cellular machines and assemblies using an integrated approach by combining three-dimensional cryo-electron microscopy (cryoEM), with biochemical, biophysical, computational and molecular biology methods. CryoEM is a powerful tool for structure determination of large protein complexes and macromolecular assemblies, and conformational changes to provide structural snapshots along dynamic processes, as well as 3D architecture of normal and disease cells under their native conditions. Current research efforts in our lab are directed to:
- HIV pathogenesis, particularly HIV capsid assembly, maturation, and interactions with host cell factors
- Molecular mechanisms of signal transduction in bacterial chemotaxis
- Developing novel technologies for single molecule imaging, including correlative light and electron microscopy, cryo-FIB/SEM of native cells, and GFP-equivalent genetically taggable EM probe for 3D localization.
We are located in the newly built Biomedical Science Tower 3 (BST3), as part of the Department of Structural Biology in the University of Pittsburgh School of Medicine. The Department fosters state-of-the-art biochemical, biophysical and structural instrumentations including X-ray diffraction, NMR and cryoEM.